Background of the study
Pathogenic microorganisms are microorganisms that can invade humans and animals and cause infections or even infectious diseases, including viruses, bacteria, fungi, rickettsiae, parasites and so on. In China, infectious diseases account for more than 50% of all diseases, and about 13 million children die of infectious diseases every year; and among the patients with infectious diseases in the clinic, 15%-60% of the population are infected with unknown pathogens, which seriously hinders the precise treatment of such diseases.
By means of a multi-omics largedata integrationThe analysis allows for more comprehensive detection of pathogenic microorganisms, understanding of the transmission mechanism and development of pathogens, thus providing the basis for the development of vaccines and antiviral drugs.
Research ideas
Since the concept of precision medicine was put forward, molecular detection technology has been increasingly used in the diagnosis and treatment of infectious diseases. Meanwhile, high-throughput molecular detection has gradually become an important research tool for pathogenic microbial infections in animal husbandry, clinical and other fields.
Next, I'll share a few case studies on the use of multi-omics techniques in pathogenic microbial infections!
Case 1
Article Topic:Multi-omics analysis of human Ebola virus pathogenesis
Journal Published: Cell Host & Microbe
Impact factor: 30.3
Technology used: transcriptomics+proteomics+ Metabolomics + Lipidomics
Overview of the study
Ebola virus (Ebola virus) is a very rare and virulent infectious virus, and the pathogenesis of Ebola Virus Disease (EVD) caused by Ebola virus is very complex. It has been shown that the pathogenesis of EVD includes high levels of viral replication and systemic dissemination, dysfunctional immune response, extensive tissue damage and organ dysfunction, and coagulation disorders, but the mechanisms of the host response that contribute to the severity of EVD are not yet understood.indefinite。
The study delved into the lethal mechanism of Ebola virus pathogenesis by combining multi-omics techniques and constructed a portfolio of biomarkers in conjunction with the multi-omics data, which could help improve the prognostic treatment of those patients at high risk.
研究思路
案例二
文章主题：宋氏鲸杆菌可提高宿主对病原体感染的抵抗力
发表期刊：Microbiome
impact factor：15.5
Technology used: genome + 16s sequencing
Overview of the study
Antibiotics are commonly used to treat pathogenic infections, but long-term use can lead to bacterial resistance as well as other health risks. Probiotics may act through four mechanisms: improvement of barrier function, immunomodulation, metabolic function, and inhibition of potential pathogens. In addition, the presence of probiotics can also reshape the intestinal native microbial community. In this study, Bacillus subtilis and zebrafish were used as probiotic and animal respectivelymould, describes the role of probiotics in modulating zebrafish intestinal commensal bacteria on host resistance to pathogen infection.
This study provides a novel mechanism by which probiotics enhance host resistance to pathogen infections in terms of their effects on the gut flora, and also demonstrates a novel function for B12 as a regulatory factor in enhancing microbial interactions. This finding may deepen the understanding of the impact of probiotics on host health and help elucidate the health benefits of vitamin B12 against pathogenic infections.
Research ideas
